Immunoblot analysis of serum reactivity against plasmid derived NiV G.

<p>Serum samples from HeV or NiV infected pigs were collected at different dpi and tested for reactivity against the homologous and heterologous recombinant protein, respectively. Porcine sera from German pigs without history of henipavirus infection served as negative control (Mock 1–3). Two German pig sera (GER11 and GER34) that exceeded the calculated cut-off value in both G based ELISAs as well as one serum (GER27) that only exceeded the cut-off value in the sHeV G based ELISA were tested exemplarily for reactivity in immunoblot analysis. All sera were diluted as indicated. The monoclonal antibody 5G1B1 was utilized in a dilution of 1:100.