Structural and functional dissection of differentially expressed tomato WRKY transcripts in host defense response against the vascular wilt pathogen (<i>Fusarium oxysporum</i> f. sp. <i>lycopersici</i>) - Fig 5

<p><b>A.</b> Histochemical staining for observation of accumulated H₂O₂ in control and <i>Fol</i> toxins exposed leaf tissues (DAB staining) at 48 hrs post inoculation of <i>Fol</i> toxins. <b>K, L and M.</b> General view of control leaf samples. <b>Q, R and S</b>. microscopic observation of control leaf tissues. <b>N and O</b>. <i>Fol</i> toxins exposed leaf tissues (attached) showing the accumulated H₂O₂ along the midrib, leaf margins and tips. <b>T and U</b>. Microscopic observation of the treated tissues accumulating higher amount of H₂O₂ along the midrib and leaf tips. <b>P and V</b>. Higher accumulation of H2O2 in leaf tissues (de-attached) <b>5B</b>. Biochemical assessment of H₂O₂ produced at different time interval. The H₂O₂ produced was higher at 24 hrs, become maximum at 48 hrs and decreases successively at increased time interval. The control tissues had more or less similar amount of H₂O₂ produced.