Responding astrocytes that form vesicles stain positive for GFAP.

<p>Observation of two sets of cells forming endocytic vesicles, highlighted by white arrows (50 minutes and 30 minutes post laser-irradiation, respectively) is displayed in rows 1 and 3. Phase contrast and corresponding immunofluorescence image following fixation and staining of previously observed cells displayed in rows 2 and 4, labeled “Post-Fix” and “GFAP”. “GFAP” immunofluorescence images are a composite of GFAP signal in green and DNA/Hoechst stain in blue.