The effect of MCU inhibition on ROS production in HepG2 cells.

<p>HepG2 cells were treated with HG for 24 h. A: H₂O₂ levels were measured using flow cytometry with DCFH-DA. B: Mitochondrial ROS level using MitoSOX red dye. Data are shown as a mean ± SEM of at least three separate experiment.: MCU knockdown cells, SC: Scramble, NG: normal glucose, HG: high glucose, # <0.05, * <0.01, * * <0.001.