Adjustment of the threshold and single cell size balance for <i>B</i>. <i>subtilis</i>.

<p>(A)—the raw image, (B) and (C)–isolated objects belonging to a given size range between 1001 and 2000 pixels, exemplified for the red (with <i>T</i> = 70) and the green (with <i>T</i> = 100) color channels, respectively. (D, F) the black curve gives the total area above the threshold <i>T</i>, which is the sum of the lower (colored) curves that represent the total area of isolated objects within a given size range for the red (D) or the green channel (F). (E, G) the black curve gives the total count of objects that appear isolated after threshold-based filtering for any given threshold value <i>T</i>, which is the sum of the lower (colored) curves that represent the total count of isolated objects within a given size range for the red (D) or the green channel (F).