Mice fed a HFD exhibit impaired muscle spindle afferent responses to stretch and vibration.

<p>Example raw and instantaneous frequency traces from a representative M CON (<b>A</b>) and M HFD (<b>B</b>) animal. Firing rates 0.5s (IST; <b>C</b>) and 3.5s (FST; <b>D</b>) into stretch were significantly decreased in both M and F mice fed a HFD. F mice had significantly lower IST and FST responses than M mice. Measures of dynamic responsiveness to stretch were also lower in HFD mice of both sexes (DP <b>E</b>; DI <b>F</b>) and there was no sex difference. Error bars denote ± SEM; *denotes p<0.05 from CON of same sex using three factor ANOVA (stretch length, diet, sex). Tables <b>G-J</b> denote percentage of afferents that can entrain to each of the 16 vibrations. Vibration amplitude increases from top to bottom and frequency increases left to right. Darker shades denote a greater percentage of entrained units. Overall, HFD animals less likely to entrain to vibration, though no difference in ability to entrain to vibration was observed between CON and HFD female mice when analyzed separately using a logistic regression model (amplitude, frequency, diet; <b>G & H</b>). Afferents from male mice fed a HFD (<b>J</b>) less likely to entrain to vibration than M CON afferents (<b>I</b>). *p<0.05 from M CON.