CD4⁺ cells from GIMAP6^fl/flCD2Cre mice show an increased size and a small increase in memory-like characteristics compared with GIMAP6 ^fl/fl mice.

<p>Panel A—The diameters of CD4⁺, CD8⁺ and B cell populations isolated from spleens of GIMAP6^fl/fl and GIMAP6^fl/flCD2Cre animals were measured using an electronic counter (CASY® Cell Counter + Analyser System—Schärfe System GMBH). Panel B–total splenic or lymph node-derived cells were stained for CD4 and then with antibodies to CD62L and CD44. Naïve cells were identified as CD62L⁺CD44^-and memory cells as CD62L^-CD44⁺. Graphs show each population expressed as a percentage of CD4⁺ cells. n = 6. P values as follows: **P<0.001 **** P<0.0001; n = 6 for each group (unpaired 2-tailed Student’s t test).