Membrane integration of the H<sub>C</sub> loop compensates for the entropic penalty inflicted by dual-receptor binding at physiological temperatures.
- Daniel Stern (213946)
- Jasmin Weisemann (5166332)
- Alexander Le Blanc (5166338)
- Laura von Berg (5166326)
- Stefan Mahrhold (5166335)
- Janett Piesker (2547574)
- Michael Laue (240602)
- Peter B. Luppa (848425)
- Martin Bernhard Dorner (5166329)
- Brigitte Gertrud Dorner (5166323)
- Andreas Rummel (469246)
Publication date
May 2018
DOIAbstract
<p><b>A</b> Representative SPR-sensorgrams for determination of thermodynamic binding parameters for H<sub>C</sub>B wild-type or the ΔH<sub>C</sub> loop mutant to isolated Syt-II, Syt-II nanodiscs, or dual-receptor nanodiscs measured at the temperatures indicated in the bar above the graphs. <b>B</b>. Thermodynamic profile depicting binding enthalpy (ΔH°), entropy (-TΔS°), and the standard Gibbs free energy (ΔG°) for those interactions; see also <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1007048#ppat.1007048.t002" target="_blank">Table 2</a> for thermodynamic data.</p
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