Supplemental Material for Kweon et al., 2018

<p><b>Figure S1</b> <i>sra</i>^KO mutant flies display normal morphology in axonal projections from PDF-expressing circadian pacemaker neurons.</p> <p><b>Figure S2</b> <i>sra</i>^KO mutation decreases PER and TIM levels in PDF-expressing circadian pacemaker neurons. Adult fly brains were dissected at ZT23 in LD cycles.</p> <p><b>Figure S3 </b>A transgenic reporter for <i>sra</i> promoter activity (<i>sra</i>-Gal4) is expressed in various brain regions of adult fly.</p> <p><b>Figure S4 </b><i>sra</i> depletion in PDF-expressing clock neurons is sufficient to dampen rhythm amplitude in free-running circadian locomotor behaviors.</p> <p><b>Figure S5 </b>Conditional depletion of <i>sra</i> in PDF-expressing neurons causes poor rhythmicity in free-running locomotor behaviors.</p> <p><b>Figure S6 </b>Genomic deletion of each calcineurin subunit weakens rhythm amplitude in free-running circadian locomotor behaviors.</p> <p><b>Figure S7 </b>Pharmacological inhibition of calcineurin reduces the steady-state levels of PER and TIM, but not CLK proteins, in <i>Drosophila</i> S2 cells.</p> <p><b>Table S1 </b><i>sra</i> mutant flies display poor rhythmicity in free-running locomotor behaviors in DD.</p> <p><b>Table S2 </b><i>sra</i> depletion in PDF-expressing clock neurons is sufficient to dampen rhythm amplitude in free-running circadian locomotor behaviors.</p> <p><b>Table S3 </b>Conditional depletion of <i>sra</i> in PDF-expressing neurons dampens free-running circadian locomotor rhythms.</p> <p><b>Table S4 </b><i>sra</i> overexpression in circadian pacemaker neurons disrupts free-running locomotor rhythms in DD.</p> <p><b>Table S5 </b>Genomic deletion of each calcineurin subunit weakens rhythm amplitude in free-running circadian locomotor behaviors. </p> <p><b>Table S6 </b>A constitutively active form of <i>Pp2B-14D</i> disrupts free-running circadian behaviors in a dose-dependent manner. </p> <p><b>Table S7 </b><i>sra</i> genetically suppresses <i>Pp2B-14D</i>^ACT to sustain circadian locomotor rhythms.</p> <p><b>Table S8 </b>Oral administration of cyclosporine A (CsA) lengthens circadian periods in free-running locomotor rhythms of wild-type flies.