Deacylation of N-linked glycans in apo-transferrin during alkaline de-N-glycosylation at 80°C for 1 h.

<p>MS spectra of indicated fractions (i–iv) in the HPLC chromatogram of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0196800#pone.0196800.g005" target="_blank">Fig 5-ii</a>. Glycan structures are depicted by using the CFG graphical notation for glycans (<a href="http://www.functionalglycomics.org/static/consortium/CFGnomenclature.pdf" target="_blank">http://www.functionalglycomics.org/static/consortium/CFGnomenclature.pdf</a>).