SET7/9 expression was elevated in methylglyoxal (MGO)-injected mice, and was associated with the level of functional impairment of the peritoneal membrane in PD patients’ effluents.

<p>(A) Immunohistochemical analyses of SET7/9 expression in peritoneal tissues of control- and MGO-injected mice (×200). (B) Numbers of SET7/9-positive (SET7/9^<b>+</b>) cells in mice with or without peritoneal MGO injection (<i>n</i> = 5 for both groups). (C) SET7/9 protein expression in nonadherent cells from human PD effluents was confirmed by Western blotting. Panel: typical results. GAPDH was used as an internal control. Full-length blots are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0196844#pone.0196844.s001" target="_blank">S1 Fig</a>. (D) Relative levels of SET7/9 protein expression. Controls: HPMCs from non-PD patients; PD patients: nonadherent cells isolated from PD effluent of patients who had undergone PD for ≥ 1 year. (E) Correlation between SET7/9 protein expression of nonadherent cells and the dialysate/plasma (D/P) ratio of creatinine (Cr) in PD patients (<i>n</i> = 12). Scale Bar = 200 μm. Data are means ± S.D. *, <i>P</i> < 0.05 (Student’s <i>t</i> test or Spearman’s rank correlation coefficient).