Inhibition of calcineurin signaling induces the expression of pro-inflammatory cytokines in keratinocytes.

<p><b>A-D</b>: qRT-PCR analysis of TNFα, IL-8 and CXCL1 expression; results were normalized to levels of 36B4 mRNA. <b>A.</b> Total mRNA isolated from mouse ears from the experiments shown in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1007366#pgen.1007366.g001" target="_blank">Fig 1A</a>; <b>B.</b> Total mRNA extracted from primary MKCs from CnB1f^flox/flox mice infected with either Ad-Cre or Ad-GFP viruses, with or without UVB treatment; <b>C.</b> Total mRNA isolated from TPA-treated HKCs transfected with siRNA of CnB1 (siCnB1) or a control scramble siRNA (siCtrl) at 24 hr; <b>D.</b> Total mRNA from HKCs treated with CsA or DSMO (Ctrl) for 24 hr, followed by treatment with TPA for another 24 hr. <b>E-G:</b> ELISA analysis of indicated cytokine protein levels in the medium. <b>E.</b> Conditioned medium collected from MKC cultures shown in <b>B</b>; <b>F,G</b>. Conditioned medium collected from HKC cultures shown in <b>C</b>. Student’s t-test was used for statistical analysis in <b>A-G</b>, standard error bars provided, * p<0.05, **p<0.01, n = 3.