Effects of 4-heptanone and SVM on nuclear localization of DAF-16::GFP in <i>C</i>. <i>elegans</i> strain TJ356.

<p>L4 larvae from strain TJ356 were treated with 4-heptanone or SVM for 24 h. Representative images of DAF-16::GFP expression in (A) control TJ356 worms and worms exposed to 4-heptanone, and SVM. Three independent experiments with at least 10 nematodes per group were performed. Scale bar = 100 μm. (B) Quantification of DAF- 16::GFP. The y-axis denotes the average number of pixels in TJ356 nematodes measured after the treatment. Mean expression ± SE from 10 nematodes is shown. Pairwise comparison of the means was performed by analysis of variance followed by post-hoc Tukey–Kramer multiple comparison test. Different letters indicate significantly different values (<i>P</i> ≤ 0.05). (C) Effect of 4-heptanone and SVM on sub cellular distribution of DAF16::GFP. Nuclear translocation of DAF16::GFP was examined in approximately 50 worms for each condition. The worms were scored as cytosolic, intermediate, and nuclear, on the basis of localization of the DAF-16::GFP. Data are presented as mean ±SEM of the percentages of each phenotype.