Shoot Regeneration from Hypocotyl and Shoot Tip Explants of Sterculia foetida L. Derived from Seedlings

Hypocotyl and shoot tip explants derived from the seedlings of Sterculia foetida were placed on MS medium supplemented with various cytokinin and auxin combinations. Cytokinin-like N6-benzylaminopurine (BAP), kinetin (KN), thidiazuron (TDZ) and auxin-like indole acetic acid (IAA), naphthalene acetic acid (NAA) and indole butyric acid (IBA) were supplemented either individually or in combinations. The regenerated shoots were separated and placed on the rooting medium augmented with different auxin-like IAA, IBA and NAA (0.1-2 mgL-1). IAA (2 mgL-1) induced 3-4 roots from the cut end of the micro shoots. Rooted plantlets were acclimatized to field conditions by placing them in pots containing sterilized sand, soil and manure mixture (1:1:1) and subsequently transferred to field with 40% survival