Additional file 3: of Estrogen-induced miR-196a elevation promotes tumor growth and metastasis via targeting SPRED1 in breast cancer

Figure S3. MiR-196b has no response to E2 treatment in ER+ BC cells. (A) ER+ BC cells MCF7 and ER- BC cells MDA-MB-231 were cultured with estrogen-free medium for 72 h before E2 treatment, then treated with 10 nM E2 or equal amount of solvent Eth as solvent control for 0, 3, 6, 12 or 24 h. The expression levels of miR-196b were analyzed by qRT-PCR and U6 levels were used as internal control, and normalized to the values of the Eth control. Data were presented as the means ± SD from three independent experiments with triple replicates per experiment. * and ** indicate significant difference compared to the 0 h group at P < 0.05 and P < 0.01, respectively. (JPEG 37 kb