Additional file 10: of Estrogen-induced miR-196a elevation promotes tumor growth and metastasis via targeting SPRED1 in breast cancer

Figure S10. The densities of CD31 levels were analyzed in tumor tissues with changes of miR-196a. MCF7/miR-196a, MCF7/miR-NC, MCF7/anti-miR-196a, or MCF7/anti-miR-NC cells were dispersed in 100 μl of serum-free DMEM medium and subcutaneously injected into the sides of posterior flank of nude mice (n = 4). The tumors were excised and sent to H&E (bar = 1000 μm) and immunohistochemistry to analyze the expression levels of CD31after 17 days. The densities of CD31 levels were quantified by ImageJ software, and presented as the means ± SD from 8 tumor tissues. (TIFF 1043 kb