<p>GST-NtCDPK1 WT, GST-NtCDPK1 S6A, and GST-NtCDPK1 T21A were autophosphorylated for the indicated time periods. The autophosphorylation state of GST-NtCDPK1 proteins was examined using Phos-tag SDS-PAGE. GST-NtCDPK1 proteins were visualized by Coomassie Brilliant Blue (CBB) staining. This experiment was repeated three times with similar results.</p
<p>(A-C) Kinase essays. Proteins were subjected to a SDS-PAGE after the kinase reaction and stained ...
<p>(A) Phosphorylation sites in each domain of BarA and ArcB are shown. (B) Autophosphorylation reac...
<p>(A) Amino acid sequence alignment showing conserved regions of the DRD2 from various species (sha...
<p>Phosphorylation of GST-NtCDPK1 proteins by His-NtCDPK1 was by reacting GST-NtCDPK1 WT, GST-NtCDPK...
<p>Autophosphorylated and unphosphorylated GST-NtCDPK1 were immobilized on glutathione beads. After ...
<p>(A) MBP-RSG was phosphorylated for the indicated time periods. Phosphorylation of Ser-114 of RSG ...
<p>Phosphorylation assays (PA) with ATPγP<sup>32</sup> were performed using NtRSG-GST (A, B) or StRS...
<p>(A) ComE is phosphorylated at a threonine residue by StkP. Top: nitrocellulose membrane stained w...
<p><b>A</b>: <i>In vitro</i> kinase assay for GST-PfCK2 autophosphorylation, top panel: autoradiogra...
<p>A, Western-blot of GST-<i>Ss</i>-RIOK-1 (89 kDa) and GST (28 kDa) are immunoprecipitated with GST...
Many protein kinases catalyze their own activation by autophosphorylation. The mechanism of this is ...
<p>(A) In vitro assay of phosphorylation by the MKK7-MPK6 cascade using wild-type GST-PIN1HL or site...
<p><b>(A) Phosphorylation of GST-p53<sup>1–64</sup> and baculovirus-expressed T-Ag by GST-wtCK1δ, GS...
<p>Drp1 is phosphorylated by GSK3beta. (A) Purified GSK3beta was used in an <i>in vitro</i> kinase a...
<p>HeLa cells stably expressing CDCP1 were left untreated or were treated for 15 minutes with 25μM p...
<p>(A-C) Kinase essays. Proteins were subjected to a SDS-PAGE after the kinase reaction and stained ...
<p>(A) Phosphorylation sites in each domain of BarA and ArcB are shown. (B) Autophosphorylation reac...
<p>(A) Amino acid sequence alignment showing conserved regions of the DRD2 from various species (sha...
<p>Phosphorylation of GST-NtCDPK1 proteins by His-NtCDPK1 was by reacting GST-NtCDPK1 WT, GST-NtCDPK...
<p>Autophosphorylated and unphosphorylated GST-NtCDPK1 were immobilized on glutathione beads. After ...
<p>(A) MBP-RSG was phosphorylated for the indicated time periods. Phosphorylation of Ser-114 of RSG ...
<p>Phosphorylation assays (PA) with ATPγP<sup>32</sup> were performed using NtRSG-GST (A, B) or StRS...
<p>(A) ComE is phosphorylated at a threonine residue by StkP. Top: nitrocellulose membrane stained w...
<p><b>A</b>: <i>In vitro</i> kinase assay for GST-PfCK2 autophosphorylation, top panel: autoradiogra...
<p>A, Western-blot of GST-<i>Ss</i>-RIOK-1 (89 kDa) and GST (28 kDa) are immunoprecipitated with GST...
Many protein kinases catalyze their own activation by autophosphorylation. The mechanism of this is ...
<p>(A) In vitro assay of phosphorylation by the MKK7-MPK6 cascade using wild-type GST-PIN1HL or site...
<p><b>(A) Phosphorylation of GST-p53<sup>1–64</sup> and baculovirus-expressed T-Ag by GST-wtCK1δ, GS...
<p>Drp1 is phosphorylated by GSK3beta. (A) Purified GSK3beta was used in an <i>in vitro</i> kinase a...
<p>HeLa cells stably expressing CDCP1 were left untreated or were treated for 15 minutes with 25μM p...
<p>(A-C) Kinase essays. Proteins were subjected to a SDS-PAGE after the kinase reaction and stained ...
<p>(A) Phosphorylation sites in each domain of BarA and ArcB are shown. (B) Autophosphorylation reac...
<p>(A) Amino acid sequence alignment showing conserved regions of the DRD2 from various species (sha...