HA binding by BM progenitors is induced by proliferation.

<p>(A) Cell cycle analysis of BM LSK cells with 7AAD labeling. Histograms showing 7AAD labeling of HA-binding and non-binding BM LSK, and percentage of cells in G0/G1 and S/G2/M phases of the cell cycle averaged from nine mice over three experiments. (B) Percentage of FL-HA binding by total LSK, and MPP and HSC sub-populations before and after <i>ex vivo</i> culture in 100 ng/ mL SCF or in 3/6/SF medium for 3 days. (C-E) BMC were isolated from the femur and tibia of uninfected control mice, or mice that were i.v. infected with 30,000 CFU of <i>Lm</i> 3, 5 or 7 days prior. (C) Number of total, MPP, or HSC in the BM of the tibia and femur from both legs of each mouse. (D) FC plots of FL-HA binding versus CD44 expression by MPP and HSC in control and infected animals 3 days post infection. (E) Percentage of FL-HA binding by MPP and HSC in control and infected animals 3, 5 or 7 days post infection. Mean +/- SD from six biological replicates of two independent experiments. *p<0.05, **p<0.01, ***p<0.001 calculated by Student’s t-test.