Entomopathogenic fungal infection results in the downregulation of PPO gene expression and reduction of phenoloxidase activity.

<p>Gene expression profiles of <b>(A)</b> <i>PPO3</i> and <b>(B)</b> <i>PPO5</i> in the midgut and fat body at 3d and 6d PI. Data represents the fold change in expression from three independent experiments. Data was analyzed by one-way ANOVA with Dunnett’s post-test. * <i>P</i><0.05, ** <i>P</i><0.01, *** <i>P</i><0.001, **** <i>P</i><0.0001. Phenoloxidase activity (Vmax) was evaluated from whole-body macerates of mosquitoes at <b>(C)</b> 3d and <b>(D)</b> 6d PI. Data represents samples from 3 independent experiments. Data was analyzed via ANOVA-Kruskal-Wallis followed by Dunn’s multiple comparison test. * <i>P</i><0.05, ** <i>P</i><0.01, *** <i>P</i><0.001, **** <i>P</i><0.0001.