Cell division patterns reveal the fate specification of early-born neurons in thalamus.

<p>(<b>A</b>) Brains were reconstructed from serial sections cumulatively containing all labeled cells within a single clone using IMARIS. Shown is a sample representative 2D projection (left panel) and 3D reconstruction (right panel) of thalamic clone derived from a <i>Gli1^CreERT2</i> MADM E18.5 brain. Violescent stereostructure sketches the contours of the hemisphere, the green filament shows the process of a residual radial glial cell, and green and red spots represent labeled GFP+ and tdTomato+ cells, respectively. Scale bar: 300 μm. (<b>B</b>) Bar graph showing the distribution of the size (cell number) of asymmetric clones. (<b>C</b>) Left: bar graph showing the average number of nuclei populated in symmetric and asymmetric G/R clones. Symmetric proliferative clones contributed to more thalamic nuclei than asymmetric neurogenic clones. Right: bar graph showing the average number of nuclei populated in majority (“major”) and minority (“minor”) hemiclones within asymmetric clones. (<b>D</b>) Bar graph showing the percentage of clones (left panel) and number of cells (right panel) populating PSN in majority (“major”) and minority (“minor”) hemiclones within asymmetric clones. dLG, dorsal lateral geniculate; eml, external medullary lamina; G, green; GFP, green fluorescent protein; LP, lateral posterior; MADM, mosaic analysis with double markers; PSN, principal sensory nuclei; PVN, paraventricular nucleus; R, red; RT, reticular nucleus; td, tandem dimer; vLG, ventral lateral geniculate; ZI, zona incerta.