Additional file 1: of Targeting chemotherapy-resistant leukemia by combining DNT cellular therapy with conventional chemotherapy

Figure S1. Gating strategy for 2 h flow-based cytotoxicity assay. Target cells were stained with PKH-26, according to manufacturer’s instructions and then effector and target cells were cultured together for 2 h before staining was performed. The co-culture was stained for CD3 and Annexin-V. Here we first gated out the debris, removed doublets, gated on the PKH+CD3− population and then determined the percentage of Annexin-V+ cells that are undergoing or having undergone apoptosis. (TIF 1571 kb