MMP-9 and MMP-2 level are up-regulated during Mtb infection or TDM induction.

<p><b>(A):</b> Heatmap showing gene expression profile generated from microarray dataset [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006974#ppat.1006974.ref034" target="_blank">34</a>] analysis comparing 5 caseous human pulmonary TB granuloma and 2 normal lung parenchyma (GSE20050). <b>(B):</b> Volcano plot showed 12878 up-regulated genes (red dots, p.value < 0.05, Fold change >2) and 8062 down-regulated genes (blue dots, p.value < 0.05, Fold change <0.5) in TB granuloma. MMP-9 and MMP-2 were labeled among the up-regulated genes. <b>(C):</b> Protein level of MMP-9 and MMP-2 in uninfected and infected mice lung (n = 3). Representative images were showed. (<b>D):</b> Representative images of luminescence from MMP-9 or MMP-2 reporter cell lines within TDM granulomas in C57BL/6J mice. The same mice were imaged at Day 3 and Day 7 (n = 5). Experiment performed twice with similar result. (<b>E):</b> Quantitative analysis of luminescent signal at Day 3 and Day 7 of MMP-2 and MMP-9 reporter cell lines within TDM granuloma (n = 5). Data represented mean ± SD. ****: p < 0.0001, 2-way ANOVA with Šidák multiple comparison test.