vFLIP interacts with CADM1.

<p>(A) HeLa cells were transfected with Flag-vFLIP. After 48 hours, cells were lysed and immunoprecipitated with either anti-Flag or control anti-IgG, followed by immunoblotting with anti-CADM1 and anti-Flag antibodies (Flag-vFLIP co-migrated with the IgG light chain). Lysates were examined for Flag-vFLIP and CADM1 expression. (B) Primary <i>Cadm1</i>^{<i>−/−</i>} MEFs were transfected with Flag-vFLIP expression vector, with or without Flag-CADM1. After 48 hours post-transfection, lysates were immunoprecipitated with anti-vFLIP and detected by immunoblotting with anti-CADM1 and vFLIP antibodies. Lysates were immunoblotted with anti-vFLIP and anti-CADM1 antibodies. (C) Lysates from PEL cell lines (BC-1, BC-3, and BCBL-1) were immunoprecipitated with either anti-CADM1 or control anti-IgG, followed by immunoblotting with anti-vFLIP and anti-CADM1. Lysates were examined for vFLIP and CADM1 expression. (D) Lysates from PEL cell lines (BC-1, BC-3, and BCBL-1) were immunoprecipitated with either anti-vFLIP or control anti-IgG, followed by immunoblotting with anti-CADM1 and anti-vFLIP. Lysates were examined for vFLIP and CADM1 expression.