The loss of Sad kinases leads to a decrease in the number of neuronal progenitors in the cortex.

<p>(A-D) Coronal sections from the brains of E13, E15 or E17 embryos with the indicated genotypes were analyzed by staining with anti-Pax6 (APCs, A, pseudo-colored cyan), anti-Tbr2 (IPCs, B, green). Nuclei were stained with Hoechst 33342 (blue). The number of Pax6⁺ (B), Tbr2⁺ (D) cells per 10⁴ μm² area in ventricular surface was quantified in the <i>Sada</i>^<i>+/-</i><i>;Sadb</i>^<i>+</i>/- (magenta) and <i>Sada</i>^<i>-/-</i><i>;Sadb</i>^<i>-</i>/- cortex (green). Values are means ± s.e.m., n = 3 different embryos, two-way ANOVA compared to heterozygous control; *, p<0.05; **, p<0.01; ****, p<0.0001). The Scale bars are 50 μm.