<i>MyoZ2</i> transcriptional activity is regulated by MEF2A and silencing <i>MyoZ2</i> inhibited myoblast differentiation.

<p>(A~B) Relative mRNA expression analysis of <i>MyoZ2</i> showed that <i>MyoZ2</i> was closely co-expressed with <i>Mef2a</i>. (C) Sequence alignments of MEF2 transcription binding site in the <i>MyoZ2</i> promoter among cattle, rat, human and mouse was highly conserved. (D) Structure of <i>MyoZ2</i> luciferase reporter vector with wild or mutant -33 bp MEF2 binding site. The mutant sequence was marked in italic red. (E) Luciferase analysis showed that MEF2A could efficiently promote <i>MyoZ2</i> transcriptional activity. (F~G) Images of immunofluorescence assay at differentiation day 6 transfected with <i>MyoZ2</i> specific siRNA or negative control siRNA (F: OLYMPUS IX71 100×; G: OLYMPUS IX71 400×). Error bars represent s.e.m. *<i>P</i> < 0.05; **<i>P</i> < 0.01.