MEF2A knockdown inhibits myoblast differentiation.

<p>(A) Morphological changes of differentiating bovine skeletal primary myoblasts at 2-day (D2), 4-day (D4) and 6-day (D6) after infection of sh-2A and sh-NC (OLYMPUS IX71 40×) (B) Images of immunofluorescence assay at differentiation day 6 treated with sh-2A or sh-NC (OLYMPUS IX71 100×). DAPI staining is shown in blue and α-actinin is shown in red. (C) Relative mRNA expression levels of myoblast differentiation marker genes (<i>MyoD</i>, <i>Mrf4</i>, <i>MyoG</i> and <i>Myh1</i>) significantly reduced in sh-2A treated cells. Error bars represent s.e.m. *<i>P</i> < 0.05; **<i>P</i> < 0.01.