MiR-425 and miR-155 produced an additive decrease in cGMP levels relative to either miRNA alone.

<p>Cardiomyocytes were transfected with 1 nM (A) or 5 nM (B) of negative control miRNA (NC), miR-425, miR-155, or the combination of miR-425 and miR-155, each at half the concentration of the other miRs. Cyclic GMP levels were measured in NPR1-expressing cells that were incubated for 2 hours with media collected from the cardiomyocytes. Cyclic GMP levels were expressed as picomoles of cGMP per mg of protein and were shown relative to levels in cells exposed to media from cardiomyocytes transfected with negative control miRNA. *<i>P</i><0.01 and **<i>P</i><1x10^-6 versus cells transfected with negative control miRNA. N = 3 experiments (4–12 replicate wells for each condition) for (A) and n = 8 experiments (4 replicate wells per condition) for (B).