In vitro evaluation of visible, near - and far infrared light radiation on cancer and normal cells

There is strong evidence that the changes in energy states of bio-molecules, induced by applied electromagnetic radiation (EMR), can lead to changes in particular biological processes [1, 2]. In this study we investigated experimentally the hypothesis of the Resonant Recognition Model (RRM) that the selectivity of protein activities is based on specific resonant electromagnetic interactions [3]. The RRM theory proposes that an external electromagnetic field at a particular activation frequency would produce resonant effects on the biological activity of a particular protein, and this activation frequency can be determined computationally [3]. In our previous study [1], it was proposed that the wavelengths of the EMR in the range of 3500-4200nm are expected to affect biological activity of proto-oncogenic proteins [1, 4]. Thus, an exposure system based on Infrared Light Emitting Diodes (IR-LEDs) was constructed and used to irradiate mouse melanoma (B16F0) and Chinese Hamster Ovary (CHO) cell lines with the computationally determined far infrared wavelengths of 3400nm, 3600nm, 3800nm, 3900nm, 4100nm and 4300nm specific to mouse cells. In addition, the same cells, B16F10 and CHO, were exposed to visible and near infrared light radiation at the wavelengths of 466nm, 585nm, 626nm, 810nm, 850nm and 950nm. The results discussed here are obtained from lactate hydrogenate (LDH) cytotoxicity assay for all twelve studied wavelengths of visible and infrared light. A qualitative analysis has also been conducted using Light microscopy and the results from this study are presented and discussed