Influence of main factors (enrichment, sex, cohort) on mean of parameters in 2 mouse strains.

<p>(A) The heatmap shows the results for all metric parameters (rows) and influencing factors and their double interactions (columns). The B6 and D2 data are on the left and right of each column, respectively. The influence of main factors was evaluated using linear models. A variable selection using the BIC was performed. Reference categories are enrichment none, sex female, and the first cohort. The differences between means of parameters are expressed as color-coded estimators β of the respective model in percent of the intercept of the model. The estimator β describes the change induced by the respective factors. The intercept is the mean of the parameter of the reference group and is set as 100%. Grey shade: variable selection using the BIC yielded nonrelevant influencing factor, thus no effect can be assumed; violet shade: factor is relevant for the model, i.e., mean is increased compared to reference group; yellow shade: factor is relevant for the model, i.e., mean is decreased compared to reference group; strength of color reflects size of difference. “X” marks parameters that were not analyzed. (B) Color grading scheme: the color grading illustrates the change in percent of the intercept; the strength of color reflects the size of difference. ABR, auditory brain stem response; ALAT/GPT, Alanine aminotransferase/Glutamat pyruvat transaminase; ALP, Alkaline phosphatase; ASAT/GOT, Aspartate aminotransferase/Glutamat oxalacetat transaminase; ASR, acoustic startle response; AU400, name of clinical chemistry analyzer; AUC, area under the curve; BIC, Bayesian Information Criterion; BN, background noise; CNPG3, substrate of test reaction (2-Chloro-4-Nitrophenyl-α-D-Maltotriosid); DEXA, dual-energy x-ray absorptiometry; HR, heart rate; HRV, heart rate variability; IgE, immunoglobulin E; IpGTT, intraperitoneal glucose tolerance test; LDH, Lactat-dehydrogenase; LVIDd, left ventricular end-diastolic internal diameter; LVIDs, left ventricular end-systolic internal diameter; LVPW, left ventricular posterior wall; P1-P4, startle amplitude following pulse of 67 (P1), 69 (P2), 73 (P3), 81 (P4) dB; PP (67, 69, 73, 81), startle amplitude and PPI following startle pulse (110 dB) with preceding pre-pulse of 67, 69, 73, 81 dB; PPI, pre-pulse inhibition; qNMR, quantitative nuclear magnetic resonance; QTc, corrected QT; R1, region analyzed (whole body excluding the skull); SHIRPA, Smithkline Beecham, MRC Harwell, Imperial College, the Royal London Hospital Phenotype Assessment; ST 110, acoustic startle response at 110 dB.