Survival curves and antibody titers in mice inoculated with RV vectors before LCMV-WE infection.

<p>(A) RV inoculation, challenge, and blood collection schedules in C57BL/6 mice. Mice were inoculated twice with RVΔP-LCMV/GPC, RVΔP, UV-irradiated RVΔP-LCMV/GPC, or PBS (mock-inoculated control) at 1-week intervals. One week after the last inoculation of RVs, mice were challenged with 10 PFU of LCMV-WE and observed for 2 weeks. Serum was collected from mice inoculated with RVs 1 day before the first RV inoculation (Pre) and second RV inoculation (1st) and at the LCMV challenge (2nd). Serum was collected from surviving mice on the last day of the 2-week observation period (Post). (B) The survival curve of mice inoculated with RVΔP-LCMV/GPC, RVΔP, UV-irradiated RVΔP-LCMV/GPC or PBS. Asterisks indicate a significant difference (p < 0.001). The titers of IgG antibody against the LCMV-GPC (C) and NAb against LCMV (D) and RV (E) in serum of mice inoculated with RVΔP-LCMV/GPC (n = 17), RVΔP (n = 13), UV-irradiated RVΔP-LCMV/GPC (n = 10), or PBS (n = 10).