MOESM7 of Deletion of HP1γ in cardiac myocytes affects H4K20me3 levels but does not impact cardiac growth

Additional file 7: Fig. S5. (A) No difference in DNA damage marker in HP1γ KO CMs. Nuclear extracts were prepared from purified CM at 8wk and WB was performed using specific antibody against γH2AX. As a positive control of γH2AX induction, C2C12 cells were treated with doxorubicin (1 μM) for 6 h. Representative pictures of 5 biologically independent experiments are shown here. (B and C) No induction of cell cycle inhibitors was seen. Gene expression of p21 (marker for DNA damage and senescence) and p16 (marker for senescence) were measured by qPCR. p21 gene expression is normalized by S26 expression. RNA from irradiated mouse embryonic fibroblasts (iMEF) was used for p16 positive control. p16 gene expression is shown with Ct value of qPCR. p16 was undetectable in both control and HP1γ KO CMs (n = 4–5)