Concentration-dependent inhibition of TRPV4 effect on Ca²⁺ response.

<p>Representative Ca²⁺-Influx measurement in NCI-H292 cells stimulated with the TRPV4 agonist GSK1016790A (2 nM) and challenged against different concentration of the TRPV4 antagonist GSK2193874 (0.1 nM, 0.3 nM, 1 nM, 3 nM, 10 nM, 30 nM, 100 nM and 300 nM) preincubated for 15 min before agonist addition in the FLIPR^TETRA. Concentration-dependent inhibition of the agonist effect through TRPV4 antagonism with an IC50 of 48,67 nM. Data are mean ± SEM; n = 3.