TRPV4 antagonist effect on ventilation induced cytokine release and protein concentration in BALF.

<p>Balb/c mice were anesthetized and mechanically ventilated (VT) in presence or absence of the TRPV4 antagonist GSK2193874 [90mg/kg] with different ventilation protocols; with tidal volumes of 20 ml/kg and 30ml/kg with a frequency of 75/min and a control group (ctrl) ventilated with a normal tidal volume of 6.5ml/kg and a frequency of 150/min for 3h and a non-ventilated control group (ctrl). (A) Increased protein concentration in BALF was observed at 30 ml/kg ventilation that could be blocked by TRPV4 inhibition (ant). (B,C) Increase release of the cytokine KC/GRO and IL-6 after a 30 ml/kg ventilation that could be blocked by TRPV4 antagonism (ant). Data are mean ± SEM; (n = 8); *p ˂ 0.05; ***p ˂ 0.001 vs 30 ml/kg VT control; one-way ANOVA Tukey's multiple comparisons test).