Cancer cell lines significantly diminishes PDL1-dependent proliferation of allogeneic and autologous CD4⁺ T cells and significantly diminishes the frequency of Granzyme A⁺ CD8⁺ T cells.

<p>Human monocytes were cultured in media alone, or with CMFDA-labeled MDA-MDA or live mf of <i>Brugia malayi</i>, for 48hr. Cells were harvested and CD45⁺/CMFDA^- monocytes were sorted and co-cultured with CFSE-labeled A) allogeneic or B) autologous lymphocytes in the presence of soluble anti-CD3 (10ug/ml) for an additional 4 days. Percent proliferation of CD4⁺ and CD8⁺ T cells was measured by flow cytometry (n = 7) either A and B) in the absence of antibody or C) in the presence of isotype control (closed circle) or anti-PDL1 (open circle). Each line represents an independent donor. *, <i>P</i> < 0.05. D) Frequency and MFI of allogeneic Granzyme A⁺ CD8⁺ T cells was measured by flow cytometry. The data are expressed as geometric mean of percent decrease in frequency and MFI of Granzyme A⁺/CD8⁺ T cells. ND = No Decrease; *<i>P</i> < 0.05. E) One representative set (n = 7) of flow histograms demonstrating proliferation of allogeneic CD4⁺ T cells either without antibody (first panel), or in the presence of isotype control or α-PDL1 (second and third panels).