CD4⁺ T-Cell subset percentages in SHIV⁺, cART suppressed animals before and after CCR5 gene editing transplantation.

<p>Upper (duodenum/jejunum; panels <b>A, C, E</b>) and lower GI biopsies (colon; panels <b>B, D, F</b>) were collected from infected and suppressed animals in Groups B-C (“ΔCCR5 Transplant,” open circles), and compared to untransplanted animals in Groups D-E (“Control,” closed circles), and Group F animals that were transplanted with non-edited HSPCs (“wt CCR5 Transplant,” gray circles). Shown are total CD3⁺CD4⁺ cells (panels <b>A-B</b>), Central Memory CD4⁺ T-cells (T_CM, panels <b>C-D</b>), and Effector memory CD4⁺ T-cells (T_EM, panels <b>E-F</b>) measured by flow cytometry from enzymatically dissociated specimens. Memory subsets were distinguished on the basis of CD45RA and CCR7 expression (see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1006956#sec002" target="_blank">materials and methods</a>). Dotted line separates pre-transplant and post-transplant time points. Exact p-values are shown where applicable.