Transmission of CHIKV (E1-226V) in <i>Aedes albopictus</i> populations from Central, Southern, and Island Italy.

<p>(A) Mosquito saliva samples were collected at 0, 2, 3, 7, and 12 days post exposure (d.p.e.) to an infectious CHIKV blood meal. Population transmission rate (PTR) and the viral mean titer, calculated by qRT-PCR and expressed in PFUeq/mL, were analyzed for all collection time; the number within the grey columns (in square bracket) represent the number of tested mosquitoes analyzed for the presence of the virus in the saliva. (B) The cumulative transmission rate (TR) and PTR of analyzed <i>Ae</i>. <i>albopictus</i> populations were calculated from 3 to 12 d.p.e. TR corresponding to the proportion of mosquitoes with positive saliva with respect to the number of mosquitoes with positive body <i>[+S/+B]</i>; PTR corresponding to the proportion of mosquitoes with positive saliva among the total number of tested fed mosquitoes <i>[+S/tested]</i>.