Potential of the studied strains to produce ATP.

<p>(A) Robustness analysis to evaluate the maximal flux of the ATPM reaction and the maximal ATP production at different growth rates. The growth rates were varied from 0.1 to 0.2 h^-1. The red lines and arrows indicate the values obtained for the experimentally measured growth rate. The red circles resulted from the interpolation with the simulated values. For these analyses we used the experimental acetate uptake rate of each strain. (B). Allocation of the total ATP production (mmol × gDW^-1 × h^-1) after constraining the respective models with the physiological parameters measured for each strain. The ATP production is shown in two portions: The non-growth associated maintenance requirement (ATPM) and the growth-associated ATP (ATPμ). The latter includes the growth associated maintenance requirement and the ATP used by some pathways (e.g. gluconeogenesis). Fluxes in A and B were calculated from optimal flux distributions of each strain by using the iterative optimization method.