In silico simulation reveals the importance of the initial basalward nucleokinesis for well-organized pseudostratification.

<p>(A) Simulation of a single clone from S phase (0 min, white) to M phase (108 min, yellow), and further to the two daughter cells’ basal nucleokinesis (until 894 min, initially green during G1, turning white [entered S phase] and orange [exited from cell cycle = differentiated]) (see also <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2004426#pbio.2004426.s004" target="_blank">S4A Fig</a> and <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2004426#pbio.2004426.s027" target="_blank">S23 Movie</a> [left]). (B) Horizontal assembly of single IKNM-simulated clones results in the formation of highly pseudostratified virtual neuroepithelium (see also <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2004426#pbio.2004426.s027" target="_blank">S23 Movie</a> [right]). (C and D) Graphs comparing the trajectories of nuclei/somata between simulation (C, <i>n</i> = 60) and real observation (D, <i>n</i> = 60) (see also <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2004426#pbio.2004426.s004" target="_blank">S4B–S4G Fig</a>). (E–G) In silico removal (from t = 0 min) of the initial basalward propelling force (illustrated in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2004426#pbio.2004426.s004" target="_blank">S4A Fig</a>, also visualized in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2004426#pbio.2004426.s004" target="_blank">S4G Fig</a>, see <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2004426#sec011" target="_blank">Materials and methods</a> for details) resulted in unsuccessful basal displacement of early G1 daughter cells’ nuclei/somata from the subapical space (E) and disruption of the virtual neuroepithelial structure (F and G; nuclear density in bin 10 is abnormally raised). Underlying data can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2004426#pbio.2004426.s031" target="_blank">S1 Data</a>. IKNM, interkinetic nuclear migration.