Inhibiting cell migration and cell invasion by silencing the transcription factor ETS-1 in human bladder cancer

As one of the members of the ETS gene family, the transcription factor v-ets avian erythroblastosis virus E26 oncogene homolog 1 (ETS-1) plays key role in the regulation of physiological processes in normal cells and tumors. In this study, we aimed to investigate the relationship between the transcription factor ETS-1 and malignant phenotypes of bladder cancer. We demonstrated that ETS-1 was up-regulated in human bladder cancer tissue compared to paired normal bladder tissue. In order to evaluate the functional role of ETS-1 in human bladder cancer, vectors expressing ETS-1 shRNA and ETS-1 protein were constructed in vitro and transfected into the human bladder cancer T24 and 5637 cells. Our results showed that the transcription factor ETS-1 could promote cell migration and cell invasion in human bladder cancer, without affecting cell proliferation and apoptosis. In conclusion, ETS-1 plays oncogenic roles through inducing cell migration and invasion in human bladder cancer, and it can be used as a therapeutic target for treating human bladder cancer.National Key Basic Research Program of China (973 Program) [2014CB745201]; Chinese High-Tech (863) Program [2014AA020607]; National Natural Science Foundation of China [81402103]; International S & T Cooperation program of China (ISTCP) [2014DFA31050]; National Science Foundation Projects of Guangdong Province [2014A030313717]; Shenzhen Municipal Government of China [ZDSYS201504301722174, JCYJ20150330102720130, GJHZ20150316154912494]; Special Support Funds of Shenzhen for Introduced High-Level Medical TeamSCI(E)ARTICLEpony8980@163.com1825125-25134