Selective demineralisation of dentine extrafibrillar minerals-A potential method to eliminate water-wet bonding in the etch-and-rinse technique

Objective: The present study tested the central hypothesis that selective demineralisation of dentine extrafibrillar minerals by lowering the phosphoric acid concentration improves the quality of the resin-dentine interface. Methods: Dentine surfaces were etched with different concentrations of phosphoric acid (1, 5,10, 20, 30 or 40 wt%). Scanning electron microscopy was used to observe the micromorphology of the etched dentine surfaces. Energy dispersive X-ray analysis was performed to determine the residual Ca-content of the demineralised dentine matrix. Atomic force microscopy-based nanoindentation was used to analyse the nanomechanical properties of the treated dentine surfaces. The influence of H3PO4 concentration on resin-dentine bond strength was evaluated by microtensile bond strength testing. One-way ANOVA was used to compare the residual Ca-content ratio, reduced elastic modulus (Er) of the treated dentine surfaces and microtensile bond strength among groups. Results: Collagen fibrils appeared to be wider in diameter after etching with 5% and 10% H3PO4. The partially-demineralized collagen scaffold retained part of its rigidity to maintain an uncollapsed three-dimensional structure. Etching with 1% H3PO4 resulted in the highest residual Ca-content ratio and Er of demineralised dentine matrix, followed by 5% H3PO4. Those values were all significantly higher than values derived from the other groups. Etching with 30% H3PO4 resulted in the lowest Ca-content ratio and Er. Using 5% H3PO4 as etchant resulted in the highest resin-dentine bond strength. Conclusions: Selective demineralisation of the dentine matrix may be achieved by lowering the H3PO4 concentration to 5 wt%, to achieve better bonding performance. Clinical relevance: By retaining intrafibrillar minerals, more through air-drying of the partially demineralised collagen matrix may be accomplished without the need to worry about collapsing a mineral-free collagen matrix during air-drying. This may result in the elimination of water-wet bonding during the application of etch-and-rinse adhesives. Published by Elsevier Ltd.National Natural Science Foundation of China [81200807]; Beijing Natural Science Foundation [7162204]; Science Foundation of Chinese Stomatological Association [CSA-B2015-08]SCI(E)PubMedARTICLEdentistzjf@163.com; tanwume@vip.sina.com; ftay@augusta.edu55-625