Relationship between Helicobacter pylori infection and miR-24-3p expression during the development of gastric mucosa lesions

目的 观察微小RNA-24-3p(miR-24-3p)在胃黏膜病变演化过程中的表达变化,并分析其与幽门螺杆菌(H.pylori)感染的关系.方法 收集2005年9月至2012年6月航天中心医院消化科行胃镜检查的患者的石蜡包埋胃组织样本共158例,根据组织形态学检查分为4组:慢性浅表性胃炎(CSG)35例、慢性萎缩性胃炎(CAG)43例、肠上皮化生(IM)41例、异型增生(Dys)39例;用快速尿素酶试验及Warthin-Starry银染检测H.pylori感染;用原位杂交方法检测各组miR-24-3p的表达.结果 miR-24-3p在CSG、CAG、IM、Dys中的表达率分别为80.0%(28/35)、39.5%(17/43)、36.6%(15/41)、38.4%(15/39),CSG组中miR-24-3p的表达率明显高于其他各组(P<0.01).H.pylori阳性的CSG标本中,miR-24-3p的表达率较相应H.pylori阴性标本显著降低(P=0.001),在其他各组中miR-24-3p的表达率与H.pylori感染状态无相关性(均P>0.05).结论 miR-24-3p在胃黏膜病变演化早期阶段高表达,并随胃黏膜病变的进展而降低;H.pylori在胃黏膜病变演化的早期阶段抑制了miR-24-3p的表达.Objectives To explore the expression of micro RNA-24-3p ( miR-24-3p) in different gastric mucosa lesions , and analyze the potential correlation between Helicobacter pylori ( H.pylori) infection and miR-24-3p expression in different gastric lesions .Methods 158 gastric biopsy specimens were divided into four groups, including 35 chronic superficial gastritis ( CSG) samples, 43 chronic atrophic gastritis (CAG) samples, 41 intestinal metaplasia (IM) samples and 39 dysplasia (Dys) samples.Those samples were collected from patients undergoing gastroscopy at the Department of Gastroenterology , Aerospace Center Hospital, from September 2005 to June 2012.The expression of miR-24-3p was detected using in situ hybridization.H.pylori infection status was determined by rapid urease test and Warthin-Starry stain. Results Higher expression rate of miR-24-3p was observed in CSG compared with those in CAG , IM and Dys, respectively.The miR-24-3p expression rate in CSG with H.pylori infection was significantly lower than that without H.pylori infection (P=0.001), but it was not observed in CAG,IM and Dys groups (all P>0.05).Conclusions MiR-24-3p was highly expressed at the early stage of gastric mucosal lesion . Attenuation of miR-24-3p expression is associated with the development of severe gastric mucosa lesions . H.pylori may play a role in miR-24-3p regulation in the early stage of gastric mucosa lesions .北京市自然科学基金(7132207)362852-28559