Protection Effects of Tranilast on Oxazolone-Induced Rat Colitis through a Mast Cell Dependent Pathway

Background:Mast cells in the gut play an important role in the innate and adaptive immune responses which are very relevant to human inflammatory bowel disease.However,the contribution of mast cells to the development of inflammatory bowel disease is not well understood.Aims:This study aimed to determine the role of mast cells in oxazolone induced colitis and to explore whether the mast cell membrane stabilizer tranilast could ameliorate this colonic inflammation.Methods:Wild-type rats and mast cell deficient rats were sensitized and challenged with oxazolone.And then treated with tranilast after challenging.Controls were treated with saline.Results:Mast cell deficient rats presented weak response to oxazolone,while wild-type rats showed severe ulcerative colitis after stimulation with oxazolone.The mast cell deficient rats model(1.8±1.64) had significantly lower disease activity index score than that of wild-type rats(8.3±0.58),P<0.01.Tranilast could reduce the secretion of cytokines,immunoglobulins and myel...Background:Mast cells in the gut play an important role in the innate and adaptive immune responses which are very relevant to human inflammatory bowel disease.However,the contribution of mast cells to the development of inflammatory bowel disease is not well understood.Aims:This study aimed to determine the role of mast cells in oxazolone induced colitis and to explore whether the mast cell membrane stabilizer tranilast could ameliorate this colonic inflammation.Methods:Wild-type rats and mast cell defici中国毒理学会免疫毒理专业委员会1