THE APPLICATION OF PATCH-CLAMP RECORDINGS IN THE CHANGE OF DORSAL ROOT GANGLION NEURONS ELECTROPHYSIOLOGICAL PROPERTIES OF CHRONIC BONE CANCER PAIN

目的：以骨癌痛大鼠为例，探讨全细胞膜片钳技术在慢性痛研究中的应用。方法：急性分离背根神经节（dorsal root ganglion, DRG）神经元，利用全细胞膜片钳技术分别记录对照组和骨癌痛组大鼠小直径DRG神经元的动作电位及TRPV1（transient receptor potential vanilloid channel subfamily V member 1）的电流，分析大鼠小直径DRG神经元电活动的变化。结果：利用全细胞膜片钳技术可以记录到骨癌痛大鼠的小直径DRG神经元静息膜电位绝对值降低、动作电位发放频率增加、爆发动作电位的阈值降低、TRPV1通道的电流幅度增大。结论：全细胞膜片钳技术可以有效而直观的记录到单个神经元动作电位和电流的变化，并由此对神经元电生理学特性进行分析，从而进行慢性痛发病机制的研究。Objective:To investigate the application of whole-cell patch-clamp recordings in chronic bone cancer pain research.Methods:Analyses of the properties of action potential and capsaicin induced inward current with whole-cell patch-clamp recordings was used in acute dissociated small DRG neurons in bone cancer pain rats and control rats.Results: The number of spontaneous and evoked action potentials of DRG neuron is more and the threshold of evoked action potentials of DRG neuron is lower in bone cancer rats than that of PBS rats, meanwhile DRG neurons depolarize in bone cancer rats compare to DRG neurons in PBS rats. Amplitude of capsaicin induced TRPV1 current is bigger in bone cancer pain rats than that of control rats.Conclusion:Whole-cell patch-clamp technology can be routinely used to characterize action potentials and current flow through ionic channels in research of mechanism of chronic pain.国家自然科学基金(81671085,81371237,61527815,31171063)；科技部国家重点基础研究发展计划“973”资助项目(2013CB531905)；卫生部行业专项基金(201302013-01)；中国中医科学院针灸研究所基础研究开放课题项目中国科学引文数据库(CSCD)125-282