Kicking down the ladder: adding cleavable features to genetically encoded photocrosslinkers

<正>Protein-protein interactions(PPIs)play vital roles in biological processes.However,the discovery of transient and biologically relevant PPIs remain a formidable challenge using conventional strategies such as co-immunoprecipitation(CoIP),yeast two hybrids(Y2H)and Forster resonance energy transfer(FRET)[1].Genetically encoded photocrosslinkers have recently emerged as a powerful approach for probing intracellular PPIs with the capability for in situ studies,low perturbation to cells as well as the wide geNational Key Research and Development Program [2016YFA0501500]; National Natural Science Foundation of China [21225206, 21432002, 21521003]SCI(E)ARTICLE2167-1696