LncRNA H19 regulates ID2 expression through competitive binding to hsa-miR-19a/b in acute myelocytic leukemia

Acute myelocytic leukemia (AML) is the most common type of acute leukemia. Long non-coding RNAs (lncRNAs) serve an important role in regulating gene expression through chromatin modification, transcription and post-transcriptional processing. LncRNA H19 was considered as an independent prognostic marker for patients with tumors. The expression of lncRNA H19 was identified to be significantly upregulated in bone marrow samples from patients with AML-M2. Furthermore, it was demonstrated that the knockdown of lncRNA H19 resulted in increased expression of hsa-microRNA (miR)-19a/b and decreased expression of inhibitor of DNA binding 2 (ID2) in AML cells. The knockdown of lncRNA H19 inhibited the proliferation of AML cells in vitro, which could be partially reversed by ID2 overexpression. Furthermore, the results of the bioinformatic analysis revealed potential hsa-miR-19a/b-3p binding sites in lncRNA H19 and ID2. Altogether, the results of the present study suggest that lncRNA H19 regulates the expression of ID2 through competitive binding to hsa-miR-19a and hsa-miR-19b, which may serve a role in AML cell proliferation.National Natural Scientific Foundation of China [81402600, 81670175]; Science and Technology Development Fund Project of Shenzhen [JCYJ20150403110829622, JCYJ20160427185541943]SCI(E)ARTICLE33687-36931