A general approach for patterning multiple types of cells using holey PDMS membranes and microfluidic channels

A novel method combining microfluidic channels and holey poly(dimethylsiloxane) PDMS membranes is presented for patterning multiple types of cells on a large variety of substrate, including substrate bearing micro- and nanometer-scale structures. Different kinds of cell are patterned on diverse flat substrates composed of various materials. Further, different cells are patterned on substrate with microgrooved substrate, and the influence of cellcell and cellsubstrate interactions about cell group behaviors is shown. This method provides a new approach for studying many processes in vitro caused by cellcell and/or cellsubstrate interactions. In addition, the method is straightforward, easy to access, convenient, and may be useful for a broad set of biological studies. ? 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.EI213715-37202