Study of compound bismuth and magnesium granules on clearance of helicobacter pylori infection in KM mice

Aim: To investigate the eradication therapy, mucosal protective effect and its mechanism of compound bismuth and magnesium granules on Helicobacter Pylori (H. pylori) infection in KM mice. Methods: HP infection model in KM mice was established by gavage with HP standard strain NCTC 11637. KM mice were randomly divided into five groups: the normal control group, the model group, the compound bismuth and magnesium granules, the PPI triple therapy group, the PPI triple therapy + compound bismuth and magnesium group. After administration for two weeks, the gastric antrum tissues were taken for rapid urease test and Warthin Starry silver stained to determine HP infection. The gastric mucosal injury and changes of cell ultrastructure were observed by HE staining and electron microscopy. Immunohistochemistry was used to detect the protein expression of IL-8, IL-10, TNF-alpha and VEGF. Results: The clearance rates in each treatment group were significantly higher than those in the model group. At the same time, there had varying degrees of improvement on inflammation of the gastric mucosa in the treatment groups compared with that in the model group. The IL-8 and TNF-alpha expression in each treatment group were lower than those in the injury group. The VEGF and IL-10 expression in each treatment group had no significant decrease. Conclusion: Compound bismuth and magnesium granules had cleaning effect on HP infection in KM mice and therapeutic effect on HP-induced gastric mucosal injury. The mechanism may be associated with inhibition of related inflammation factors.Beijing Medical Association Research Fund [2012-001]SCI(E)ARTICLEdjjyhu@163.com; ygb@medmail.com712888-12895