Carbon nanotube-assisted optical activation of TGF-beta signalling by near-infrared light

Receptor-mediated signal transduction modulates complex cellular behaviours such as cell growth, migration and differentiation. Although photoactivatable proteins have emerged as a powerful tool for controlling molecular interactions and signalling cascades at precise times and spaces using light, many of these light-sensitive proteins are activated by ultraviolent or visible light, which has limited tissue penetration. Here, we report a single-walled carbon nanotube (SWCNT)-assisted approach that enables near-infrared light-triggered activation of transforming growth factor beta (TGF-beta) signal transduction, an important signalling pathway in embryonic development and cancer progression. The protein complex of TGF-beta and its latency-associated peptide is conjugated onto SWCNTs, where TGF-beta is inactive. Upon nearinfrared irradiation, TGF-beta is released through the photothermal effect of SWCNTs and becomes active. The released TGF-beta activates downstream signal transduction in live cells and modulates cellular behaviours. Furthermore, preliminary studies show that the method can be used to mediate TGF-beta signalling in living mice.National Basic Research Program of China (973 program) [2012CB917303]; National Natural Science Foundation of China [91313301, 21425204, 91127034]SCI(E)ARTICLExingchen@pku.edu.cn5465-4711