Experience-Dependent Plasticity of Dendritic Spines of Layer 2/3 Pyramidal Neurons in the Mouse Cortex

Previous studies have shown that sensory and motor experiences play an important role in the remodeling of dendritic spines of layer 5 (L5) pyramidal neurons in the cortex. In this study, we examined the effects of sensory deprivation and motor learning on dendritic spine remodeling of layer 2/3 (L2/3) pyramidal neurons in the barrel and motor cortices. Similar to L5 pyramidal neurons, spines on apical dendrites of L2/3 pyramidal neurons are plastic during development and largely stable in adulthood. Sensory deprivation via whisker trimming reduces the elimination rate of existing spines without significant effect on the rate of spine formation in the developing barrel cortex. Furthermore, we show that motor training increases the formation and elimination of dendritic spines in the primary motor cortex. Unlike L5 pyramidal neurons, however, there is no significant difference in the rate of spine formation between sibling dendritic branches of L2/3 pyramidal neurons. Our studies indicate that sensory and motor learning experiences have important impact on dendritic spine remodeling in L2/3 pyramidal neurons. They also suggest that the rules governing experience-dependent spine remodeling are largely similar, but not identical, between L2/3 and L5 pyramidal neurons. (C) 2015 Wiley Periodicals, Inc.Peking University Shenzhen Graduate School; National Institutes of Health [P01 NS074972, R01 GM107469, R21 AG048410]; Whitehall Foundation; National Natural Science Foundation of China [81100839]; Shenzhen Science and Technology Innovation Funds [GJHS20120628101219327]SCI(E)PubMedARTICLEgan@saturn.med.nyu.edu3277-2867