Preparation and evaluation of lipidmatrix nanocarrier codelivery gene and sensibilizer to elevate docetaxel antitumor

It is a promising treatment strategy to use a nanoparticlebased drug delivery system for cancer patients, which can simultaneously deliver multiple drugs or genes in combination with therapy to induce synergistic effects and suppress drug resistance to the tumor. In this study, cationic nanostructured lipid carriers (cNLC) for coloading anionic smallinterfering RNAs (siRNA) and chemotherapeutic docetaxel (DTX) were prepared from different cationic lipids based on particle distribution and loading efficiency. In order to increase the cNLC’s positive targeting capacity, a novel peptide SP94 was bound to the surface of cNLC (SP94cNLC). The cNLC showed good efficiency in loading siRNA and DTX. The SP94cNLC revealed a better cytotoxicity compared with cNLC and Taxotere~?, indicating that SP94 could successfully enhance the internalization capacity of nanoparticles to the liver cancer cells. This new type of cNLC is a potential vehicle when using in codelivery of chemotherapeutics and siRNAs. The curcumin (CUR)/DTX codelivery NLC could load both CUR and DTX in high efficiency and showed a sensibilization to DTX chemotherapy. The sensibilization was more obvious when it was used in the aggressive and resistant cancer cells. This CUR/DTX codelivery system had good potential in treating cancer cells when chemotherapy drug showed little effect alone.National Natural Science Foundation of China,Beijing Natural Science Foundation,Doctoral Foundation of the Ministry of Education中国科学引文数据库(CSCD)3145-1522